PONOMAREV Eugene D

Assistant Professor

B.S., M.S., Ph.D.

Telephone:  39439620

Email:  This email address is being protected from spambots. You need JavaScript enabled to view it.

Address:

 Room 323A, Lo Kwee-Seong Integrated Biomedical Sciences Building, Area 39, The Chinese University of Hong Kong

Website:  https://scholar.google.com/citations?user=SZDQ_oQAAAAJ&hl=en/citations?user=0jvjVzQAAAAJ&hl=en

 

  1. Our research interest is related to the immunological aspects of inflammation in the central nervous system (CNS) associated with neurodegenerative disease such as multiple sclerosis, Alzheimer’s disease and traumatic brain injury.
  2. Epigenetic and transcriptional control of microglia/macrophage activation and polarization during CNS inflammation.
  3. The role of platelets in the initiation and propagation of neuroinflammation.
  1. Dukhinova, M., Kopeikina, E. & Ponomarev, E.D. (2018). Chapter 10: Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration. Chapter in edited book: Cellular Heterogeneity. Methods and Protocols. Publisher: Springer Nature, ISBN: 978-1-4939-7679-9. (Accepted).
  2. Kopeikina, E., Dukhinova, M. & Ponomarev, E.D. (2018). Chapter 9: Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry Chapter in edited book: Cellular Heterogeneity. Methods and Protocols. Publisher: Springer Nature, ISBN: 978-1-4939-7679-9. (Accepted).
  3. Maksimova, N., Krasheninnikov, M., Zhang, Y., Ponomarev, E.D.., Pomytkin, I., Melnichenko, G. & Lyundup, A. (2017). Early passage autologous mesenchymal stromal cells accelerate diabetic wound re-epithelialization: A clinical case study. Cytotherapy. pii: S1465-3249 (17) 30691-6: doi: 10.1016/j.jcyt.2017.08.017.
  4. Kholodenko, R.V., Kalinovsky, D.V., Doronin, I.I., Ponomarev, E.D. & Kholodenko, I.V. (2017). Antibody Fragments as Potential Biopharmaceuticals for Cancer Therapy: Success and Limitations. Curr Med Chem. 24 (42): doi:10.2174/0929867324666170817152554.
  5. Strekalova, T., Pavlov, D., Markova, N, Bettendorff, L., Chekhonine, V., Pomytkin, I., Lioudynog, V., Svistunov, A., Ponomarev, E.D. & Lesch, K.P. (2017). Elucidating the functions of brain GSK3a: possible synergy with GSK3b upregulation and reversal by antidepressant treatment in a mouse model of depressive-like behaviour. Behavioural Brain Research. 335: 122-127.
  6. Barteneva, N.S., Baiken, Y., Fasler-Kan, E., Alibek, K., Wang, S., Maltsev, N., Ponomarev, E.D.., Kauanova, S., Moore, A., Beglinger, C. & Vorobjev, I.A. (2017). Extracellular vesicles in gastrointestinal cancer in conjunction with microbiota: On the border of Kingdoms. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 1868 (2): 372-393.
  7. Starossom, S.C., Veremeyko, T., Dukhinova, M., Yung, A.W.Y., Weiner, H.L. & Ponomarev, E.D. (2015). Platelets Play Differential Role in the Regulation of the Initiation and Progression of Autoimmune Neuroinflammation. Circulation Research. 117 (9): 779-792.
  8. Doronin, I.I, Vishnyakova, P.A., Kholodenko, V.I., Ponomarev, E.D., Ryazantsev, D., Molotkovskaya, I.M & Kholodenko, R.V. (2014). Ganglioside GD2 in reception and transduction of cell death signal in tumor cells. BMC Cancer. 14(1): 295.
  9. Starossom, S.C., Veremeyko, T, Dukhinova, M., Yung, A.W.Y. & Ponomarev, E.D. (2014). Glatiramer acetate (Copaxone) modulates platelet activation and inhibits thrombin-induced calcium influx: possible role of Copaxone in targeting platelets during autoimmune neuroinflammation. PLOS ONE. 9(5): e96256.
  10. Barteneva, N.S., Ponomarev, E.D., Tsytsykova, A., Armant, M. & Vorobjev, I. A. (2014). Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting. J. Histochem. Cytochem. 62(4):265-275.
  11. Veremeyko, T., Siddiqui, S., Sotnikov, I., Yung, A. & Ponomarev, E.D. (2013). IL-4/IL-13-Dependent and Independent Expression of miR-124 and its Contribution to M2 Phenotype of Monocytic Cells in Normal Conditions and During Allergic Inflammation. PLOS ONE. 8(12): e81774 (most cited paper for 2017).
  12. Barteneva, N.S., Fasler-Kan, E., Bernimoulin, M., Stern, J.N., Ponomarev, E.D., Duckett, L., Vorobjev, I.A. (2013). Circulating microparticles: square the circle. BMC Cell Biol. 14(1): 23.
  13. Sotnikov, I., Vermeyko, T., Starossom, S.C., Barteneva, N., Weiner, H.L. & Ponomarev, E.D. (2013). Platelets recognize brain-specific glycolipid structures, respond to neurovascular damage and promote neuroinflammation. PLOS ONE. 8(3): e58979.
  14. Ponomarev, E.D.., Veremeyko, T. & Weiner, H.L. (2013). MicroRNAs are universal regulators of differentiation, activation, and polarization of microglia and macrophages in normal and diseased CNS. GLIA. 61(1): 91-103.
  15. Veremeyko, T., Starossom, S.C., Weiner, H.L. & Ponomarev, E.D. (2012). Detection of microRNAs in microglia by real-time PCR in normal CNS and during neuroinflammation. J. Vis. Exp. 65: 10.3791/4097.
  16. Ponomarev, E.D., Veremeyko, T. & Barteneva, N. (2011). Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry. BMC Res Notes. 4: 517.
  17. Ponomarev, E.D., Veremeyko, T., Krichevsky, A.K. & Weiner, H.L. (2011). MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the CEBPalpha-PU.1 pathway. Nat. Med. 17:64-70.
  18. Maresz, K*, Ponomarev, E.D. *. Barteneva, N., Tan, Y., Mann, M.K. & Dittel, B.N. (2008). IL-13 induces the expression of the alternative activation marker Ym1 in a subset of testicular macrophages. J. Reprod. Immunol. 78:140-148 (*equally contributing).
  19. Ponomarev, E.D., Maresz, K., Tan, Y. & Dittel, B.N. (2007). CNS-Derived Interleukin-4 is Essential for the Regulation of Autoimmune Inflammation and Induces a State of Alternative Activation in Microglial Cells. J. Neurosci. 27: 10714-10721.
  20. Maresz, K., Pryce, G., Ponomarev, E.D., Marsicano, G., Croxford, L., Shriver, L., Ledent, C., Cheng, X., Carrier, E., Mann, M.K., Giovanni, G., Pertwee, R.G., Yamamura, T., Buckley, N., Hillard, C.J., Lutz, B., Baker, D. & Dittel, B.N. (2007). Direct Supression of CNS Autoimmune Inflammtion Via the Cannabinoid Receptor CB1 on Neurons and CB2 on Autoreactive T cells. Nat. Med. 3: 492-497.
  21. Ponomarev, E.D., Shriver, L.P. & Dittel, B.N. (2007). GM-CSF Production by Autoreactive T cells is required for the Activation of Microglial Cells and the Onset of Experimental Autoimmune Encephalomyelitis. J. Immunol. 181: 39-48.
  22. Ponomarev, E.D., Shriver, L.P. & Dittel B.N. (2006). CD40 Expression on Microglial Cells Is Required for Their Completion of a Two-Step Activation Process during CNS Autoimmune Inflammation. J. Immunol. 176: 1402-1410.
  23. Maresz, K., Carrier, E., Ponomarev, E.D., Hilliard, C. & Dittel, B.N. (2005). Modulation of the Endocannabinoid CB2 Receptor in Microglial Cells in Response to Inflammatory Stimuli*. J. Neurochem. 95:437-445.
  24. Ponomarev, E.D.., Shriver, L.P., Maresz, K. & Dittel, B.N. (2005). Microglial Cell Activation and Proliferation Precedes the Onset of CNS Autoimmunity. J. Neuroscience Res. 2005, 81: 374-389.
  25. Ponomarev, E.D., Maresz, K, Shriver, L.P. & Dittel, B.N. (2005). Development of a Culture System that Supports Adult Microglial Cell Proliferation and Maintenance in the Resting State. J. Immunol. Methods. 300: 32-46.
  26. Ponomarev, E.D. & Dittel, B.N. (2005). Gamma-delta T cells Regulate the Extent and Duration of Inflammation in the Central Nervous System by a Fas Ligand-Dependent Mechanism. J Immunol. 174: 4678-4687.
  27. Ponomarev, E.D., Novikova, M., Yassai, M., Szczepanik, M., Gorsky, J. & Dittel, B.N. (2004) Gamma-delta T cell regulation of IFN-gamma production by CNS infiltration encephalitogenic T cells: Correlation with disease recovery. J. Immunol. 173: 1587-1595.
  28. Sapozhnikov, A.M., Gusarova, G.A., Ponomarev, E.D. & Telford G.W. (2002). Translocation of cytoplasmic HSP70 onto the surface of EL-4 cells during apoptosis. Cell Proliferation, 35: 193-206.
  29. Ponomarev, E.D., Tarasenko, T.N. & Sapozhnikov, A.M. (2000). Splenic Murine Cytotoxic Cells Recognize Surface HSP70 on Culture-Adapted EL-4 Lymphoma Cells. Immunol. Lett.74: 133-139.
  30. Sapozhnikov, A.M., Ponomarev, E.D., Tarasenko, T.N. & Telford, G.W. (1999). Spontaneous Apoptosis and the Expression of Cell Surface Heat Shock Proteins in Cultured EL-4 Lymphoma Cells. Cell Proliferation. 32: 363-368.