BD FACSAria Fusion Flow Cytometer

BD FACSAria Fusion Flow Cytometer
Features: Fitted with blue, yellow-green, red, violet and near-UV lasers allowing 11-colour cell analysis and sorting

1. 488 nm Blue laser

Filter: FITC (530/30 nm). PE-Cy5.5 (695/40 nm)

2. 561 nm Yellow Green laser

Filter: PE / PE-mCherry (582/15 nm), PE-Texas Red (610/20 nm), PerCP-Cy5 (670/14 nm), PE-Cy7 (780/60 nm)

3. 633 nm Red laser

Filter: APC (660/20 nm), APC-Cy7 (780/60 nm)

4. 375 nm Near UV laser

Filter: Hoechst Blue (450/40 nm), Hoechst Red (670LP)

5. 405 nm Violet laser

Filter: Pacific Blue (450/50 nm), BV 605 (610/20 nm), BV 711 (710/50 nm) OR AmCyan (525/50 nm)

 

375 nm near UV and 405 Violet lasers cannot be turned on simultaneously

Staff-in-charge:

- Eric
Back-up Support: - Venus / YK Suen
Location: LIBSB 412

 

BD FACSAria II Flow Cytometer

DSC02490 F
Features: Fitted with blue, red and near-UV lasers allowing 9-colour cell analysis and sorting

1. 488 nm Blue laser

Filter: FITC (530/30 nm), PE (585/42 nm), PE-Texas Red (616/23 nm), PerCP-Cy5.5 (695/40 nm), PE-Cy7 (780/60 nm)

2. 633 nm Red laser

Filter: APC (660/20 nm), APC-Cy7 (780/60 nm)

3. 375 nm Near UV laser

Filter: Hoechst Blue (450/40 nm), Hoechst Red (670LP)

Staff-in-charge:

- Eric
Back-up Support: - Venus / YK Suen
Location: LIBSB 412

 

BD LSR Fortessa

DSC02484 F
Features: Fitted with blue, red, violet and UV lasers allowing 13-color cell analysis.

1. 488 nm Blue laser

Filter: FITC (530/30 nm), PE (575/26 nm), PE-Texas, Red (610/20 nm), PerCP-Cy5.5 (695/40 nm), PE-Cy7 (780/60 nm)

2. 633 nm Red laser

Filter: APC (670/14 nm), APC-Cy7 (780/60 nm), Alexa Fluor 700 (730/45)

3. 405 nm Violet laser

Filter: Pacific Blue (450/50 nm), AmCyan (525/50 nm), Qdot 605 (605/12 nm)

4. 355 nm UV laser

Filter: Indo-1 (violet), Hoechst Blue, DAPI (450/50 nm), Hoechst Red (670/20 nm), Indo-1 (Blue) (530/30 nm)

Staff-in-charge:

- Eric
Back-up Support: - Venus / YK Suen
Location: LIBSB 412

 

FlowJo software v10.2

FlowJo software v10.2 FlowJo software v10.22
Features:

- is an alternative software for analyzing flow cytometry data offline

- Generates high resolution publication-quality graphics, overlay pictures and tables 

- Creates Batch analysis and statistic table

- Included easy-to-use Compensation, Cell cycle analysis, Cell proliferation modules

Staff-in-charge:

- Eric
Back-up Support: - Venus / Randy
Location: LIBSB 412B