Leica TCS SP8 White Light (WLL) Confocal Microscope

IMG Core C01

Features:

White confocal combines a white light laser source, tunable excitation filtering, tunable beam-splitting and tunable emission-detection – with full access to the keys for all colors.  It allows users to specify any excitation wavelength between 470nm and 670nm.

Together with an AOBS (acoustical optical beam splitter) up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. Because the WLL system is a pulsed laser it can be used in conjunction with hybrid detectors to provide gated suppression (removal) of auto-fluorescence and reflected light. The SP8's spectral detection system uses a prism spectrometer based emission separation (not filters) that allows user selection of emission detection bandwidth as well as spectral scanning of emission profiles. This allows users to precisely select any emission band-pass they require. Additionally, the system can scan at 8000Hz for ultra-fast scanning (and greatly reduced photo-bleaching) via its selectable resonant scanner. It is fully enclosed for heating and environmental control with media perfusion capabilities and CO2 control. The system is ideal for imaging live cells, tissue in culture dishes and chambered cover glass (#1.5).

Microscope
Leica DMi8 CS Motorized inverted microscope with universal stage insert for glass slide, Petri dishes of size from 35mm to 60mm.

  • Light source EL6000
  • Filter cubes: DAPI LP, FITC LP, RHOD LP, Y5, CFP/YFP  
  • Super Z Galvo stage Type H using closed-loop control of auto-focus system / drift compensation
  • Insert Universal Type H
  • Multiwell plate insert for Super Z
  • Petri dish insert 36 mm for Super Z
  • Closed Loop Focus with AFC
  • Adaptive Focus Control – Laser based autofocusing ensures that the sample remains in focus throughout time-lapse experiment


Objectives

  • HC PL APO 10x/0.40 CS2 (FWD 2.2mm)
  • HC PL APO 20x/0.75 CS2 (FWD 0.62mm)
  • HCX PL APO 40x/0.85 CORR CS1 (FWD 0.21mm)
  • HC PL APO 63x/1.20 W CORR CS2 (FWD 0.3mm)
  • HC PL APO 63x/1.40 OIL CS2 (FWD 0.14mm)


Super Z incubation system and accessories

Tokai Hit INUG2-GSI2-SET -  Control Unit (110 - 240V)

  • GSI2 Chamber Unit(T11)
  • Temperature Controller with built-in digital gas mixer for 100% CO2 gas use. Supply fixed 5%CO2+95% Air
  • Dish Attachment
    • GSI2-D35(TD11)- Dish Attachment for 35 mm dish
    • GSI2-CGC(TD13) - Dish Attachment for chambered coverglass
    • GSI2-CS(TD14) - Dish Attachment for chamber slide and slide glass
  • Sensor Lid
    • D35-200F(TS1) - Sensor Lid for 35 mm dish
    • CSG-200F(TS14) - Sensor Lid for chamber slide and chambered coverglass


Light Sources

  • WLL2 (tunable), avg. power 1.5 mW: 470 – 670 nm, 78 MHz; with integrated pulse picker: 78, 39, 19.5, 9.75 MHz
  • UV / 405 nm laser, 50mW
  • Ar-Laser, Blue, 65 mW with following wavelengths: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • Leica EL6000 – Mercury metal halide bulb: ~ 300 nm – 700nm (peaks at 365, 405, 436, 546, 579)

An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light.

An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.


Detectors

Reflection/fluorescence mode (spectral detection range 400-800 nm)

  • 2 x HyD (GaAsP detectors - Operating mode: Standard, counting, BrightR) + time-gating
  • 2 x PMT (Standard photomultiplier tube)
  • 1 Transmitted light detector


Scanners

X2Y-scanner with optically correct scanning at low inertia 
Conventional scanner: 1-3600Hz (bidirectional)

  • Maximal frame rate 512 x 512: 7 frames/sec
  • Maximal frame rate 512 x 16: 112 frames/sec
  • Maximal frame resolution: 8192 x 8192 pixel
  • Scan zoom: 0.7 – 48 x
  • Field rotation:  220° optical
  • Field diameter: 22 mm


Resonant scanner
: 8K – 16KHz

  • Maximal frame rate 512 x 512: 28 frames/sec
  • Maximal frame rate 512 x 16: 290 frames/sec
  • Maximal frame resolution: 2496 x 2496 pixel
  • Scan zoom: 1.25 – 48 x
  • Field rotation:  200° optical
  • Field diameter: 13 mm


Scan modes

xyz, xt, xyt, xyzt, xyλ, xyλt, xyzλ, xyzλt, xzy, xzt, xzyt, xzλ, xzλt, 
xyL, xzL, xyzL, xyLt, xyλL, xzλL (WLL) 


Softwares

  • Image acquisition
  • Mosaicking / Stitching
  • Online dye separation
  • Photon statistics
  • Lambda scan
  • Lambda-lambda scan
  • LASX SP8 Control Software
  • LASX Dye Finder
  • LightGate via pulsed WLL and HyD detectors helps remove reflected light as well as autofluorescence
  • LASX Lightning
  • LASX 3D Visualization
  • LASX Co-Localization
  • LASX MicroLab
  • LASX FRAP Zoomer
  • LASX 2D analysis
  • LASX 2D Analysis Multi ChannelExtension
  • LASX Environmental Control
  • LASX Live Data mode
  • LASX SP8 Offline analysis
    • LASX Basic license, Live Data Mode, Colocalization, FRET, FRAP, Dye Finder, Electrophysiology

Staff-in-charge:

Anny Cheung
Back-up Support: Josie Lai
Location: LIBSB 108A
Remarks:

- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. 
- Data files greater than 1GB will be automatically removed TWO working days after experimentation.

 

Olympus FV1200 SIM Confocal System with ZDC

IMG Core C01

Features: - Equipped for fixed and live cell imaging, time-lapse imaging, multi-fluorescence z-stack 3-D, FRET/FRAP, SIM/HSD, multi-position time-lapse and mosaic outline imaging;
Fiber: Broadband type (400nm – 800nm)
Laser light: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW
Detector mode: 3 PMTs; 2 high sensitive GaAsPs detector with Peltier cooling
Microscope: Olympus IX83 Inverted microscope
Objectives: 4x, 10x, 20x, 30x (silicone oil), 40x, 60x (water), 60x (oil), 60x (silicone oil)
FL filters: DAPI, GFP, Cy3, Cy5
Information on Olympus IX83 FL Chroma filter sets
Motorized stages: Prior Model 117 with joystick
Incubation chamber: ibidi Heating System, Universal Fit with gas incubation system
Software: FluoView 4.2a

Staff-in-charge:

- Josie
Back-up Support: - Anny/Samuel
Location: LIBSB 108B
Remarks: For SBS Experienced and Trained user only.

- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice.

- Data files greater than 1GB will be automatically removed TWO working days after experimentation.

 

Olympus FV1000-ZCD Laser Scanning Confocal System

 IMG Core C03

Features: - Equipped for fixed and live cell imaging, time-lapse imaging and multi-fluorescence z-stack 3-D imaging;

- Connected to an IX81 inverted microscope with objectives 4x, 10x, 20x, 40x, 63x oil and epi-fluorescence filters for DAPI, FITC and TRITC;

- Incubation chamber for live cell imaging;

- Laser sets of spectral type multi-Argon (458, 488, 515) LD405, LD559 and LD635.

Staff-in-charge:

- Josie
Back-up Support: - Anny / Samuel
Location: LIBSB 108B
Remarks: For SBS Experienced and Trained user only.

- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice.

- Data files greater than 1GB will be automatically removed TWO working days after experimentation.

 

Leica SP-5 Confocal System (No UV Laser)

IMG Core C04

Features: - Equipped for fixed/live cell imaging; time-lapse imaging and high-speed multi-fluorescence optical sectioning;

- Connected to Leica DMI6000 inverted microscope with fluorescence filters for I3 (Ex 450-490 / Em LP515) and N2.1 (Ex 515-560 / Em LP590);

- Laser sets multi-Argon 458, 476, 488, 514, DPSS 561 and HeNe633;

- Objectives: 10x, 20x, 40x, 40x oil, 63x water, 100x oil.

Staff-in-charge:

- Josie
Back-up Support: - Anny / Samuel
Location: LIBSB 110
 Remarks:  - Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice.

- Data files greater than 1GB will be automatically removed TWO working days after experimentation.

 

Olympus IX83 Fully Motorized Inverted Microscope with ZDC

ix83

Features:

- Equipped for fixed and live cell imaging

 

Microscope
- Objectives:

• UPLSAPO4X, N.A. 0.16, WD 13
• UPLSAPO10X, N.A. 0.4, WD 3.1
• UPLSAPO20X, N.A. 0.75, WD 0.6
• UPLSAPO40X, N.A. 0.95, WD 0.18

Filter sets: DAPI/Hoechst, FITC/EGFP, PI/TRITC, Cy5
Information on Olympus IX83 FL Chroma filter sets

Light source: X-Cite 120Q Metal halide or CoolLED pE4000 UV with 16 Selectable wavelengths including 365, 385, 405, 435, 460, 470, 490, 500, 525, 550, 580, 595, 635, 660, 740, 770nm

Software interface: CellSens Dimension

Camera: DP80 Double-CCD


Color CCD:Resolution at 12.5 megapixels (4080 x 3072)

Monochrome CCD:
Resolution at 1.4 megapixels (1360 x 1024)
Binning: 2 x 2, 4 x 4

Staff-in-charge:

- Josie
Back-up Support: - Anny / Samuel
Location: LIBSB 110

  

Olympus SZX16 Stereomicroscope Imaging System

szx16

Features: Microscope

- Zoom range:
16.4 (0.7, 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8, 10, 11.5)

- Objectives:
1x Plan Apochromat, N.A. 0.15, W.D.(mm) 60 Total magnification 7x - 115x
2x Plan Apochromat, N.A. 0.3, W.D.(mm) 20 Total magnification 14x - 230x

Filter sets: DAPI, GFP, Cy3, Cy5
Illumination: X-Cite 120Q Metal halide or CoolLED pE4000 UV 635, 660, 740, 770nm

Software interface: CellSens

Camera: DP80 Double-CCD

Color CCD: Resolution at 12.5 megapixels (4080 x 3072)

Monochrome CCD:
Resolution at 1.4 megapixels (1360 x 1024)
Binning: 2 x 2, 4 x 4

Staff-in-charge:

- Josie
Back-up Support: - Anny / Samuel
Location: LIBSB 110

 

Imaris 7.72 by Bitplane

imaris

Features:

Imaris is Bitplane's core scientific software module that delivers all the necessary functionality for data visualization, segmentation, analysis and interpretation of 3D and 4D microscopy datasets.

 

Modules available:

  • Imaris Track
  • Imaris Measurement Pro
  • Imaris Coloc
  • FilamentTracer
  • Imaris Cell
  • Imaris Vantage
  • Imaris Batch

Staff-in-charge:

- Josie
Back-up Support: - Anny/Samuel
Location: LIBSB 108