Affymetrix Microarray System

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Features: - Equipped with Hybridization Oven 640, Fluidics Station 450 and GeneChip Scanner 3000 7G system;

- Incorporate advanced gene expression analysis and genotyping studies;

- Transcription detection e.g. coding mRNA, Copy number variation (CNV), Single nucleotide polymorphism (SNP);
- Genechips and consumables are not provided by Core Labs.

Staff-in-charge:

- YK Suen / Eric
Location: LIBSB 612

 

Partek Genomic Suite and Partek Pathway Software for Microarray data analysis

 

Features: - Partek Genomics Suite is a comprehensive suite of advanced statistics and interactive data visualization, specifically designed to reliably extract biological signals from noisy data.

- The software is unique in supporting all microarray.

Staff-in-charge:

- Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

Agilent 2100 Bioanalyzer

bioana

Features: - Serves as a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells Serves as a review control taken during the target preparation prior to committing to the hybridization of the Microarray

- Genechips and consumables are not provided by Core Labs.

Staff-in-charge:

- Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

Automated Next Generation Sequencing (NGS) System: The Ion Chef System & Ion Torrent S5 Sequencer


The Ion Chef System Ion Torrent S5 Sequencer
The Ion Chef System and the Ion S5 Sequencing System enable targeted next-generation sequencing workflow for panels, microbes, exomes, FFPE, transcriptomes and copy number analysis.

Features:

Ion Chef System

  •  The system helps to simply the next-generation sequencing workflow for Ion S5,
     
  •  It incorporates automated Ion Ampliseq library preparation, template preparation and chip loading in the system with the use of pre-packaged cartridge-based reagent kits for library preparation.
  • It enables reproducible template preparation on 2 chips for multiple samples for sequencing within hours.
  •  Ion chips (Ion 510, 520, 530 & 540 chips are compatible for use) and consumables required will not be provided by Core Labs.


Ion S5 Sequencing System

- This system has the capability to multiplex thousands of targets and hundreds of samples in a single sequencing run.

-  DNA/RNA for sequencing can be as low as 1-10ng using Ion AmpliSeq technology.


- Workflow for panels, microbes, exomes and transcriptomes on Ion S5 System:

  Ion 510 Chip Ion 520 Chip Ion 530 Chip Ion 540 Chip
Reads   2-3 million 4-6 million 15-20 million 60-80 million
Output 200 bp 0.3-0.5 Gb 0.6-1 Gb 3-4 Gb 10-15 Gb
400 bp 0.6-1 Gb 1.2-2 Gb 6-8Gb
Run times 200 bp 2.5 hr 2.5 hr 2.5 hr 2.5 hr
400 bp 4 hr 4 hr >4 hr

Analysis time

200 bp 2 hr 5 hr 8 hr 16.5 hr
400 bp 6.5 hr 8 hr 17.5 hr

-Workflow for long-read sequencing for research applications such as human leukocyte antigen (HLA) typing or metagenomic analysis on Ion S5 System

Ion 510 Chip Ion 520 Chip Ion 530 Chip Ion 540 Chip
Reads 600bp  3-4 million 9-12 million
Outputs* 600bp  0.5-1.5 Gb  1.5-4.5 Gb
Run time 600bp 4hrs 4hrs
Analysis time†† 600bp 8hrs 17hrs

 

Built-in informatics

  • Data generated will automatically be uploaded from Torrent Suite Software to Ion Reporter™ Software for further analysis.

Staff-in-charge:

- Venus
Back-up Support: - Randy/Eric
Location: LIBSB 612
Booking of use:
  • For SBS MEMBERS ONLY
  • Training is needed for all uses. Please contact Venus in person for discussion and booking.
  • Booking system will be on daily basis, from Monday to Friday ONLY.