中心實驗室成員
Managing Director and Chair, Committee on Core Laboratories Management |
Prof. TSUI Kwok Wing Stephen |
Deputy Managing Director | Dr. VONG Si Long Joaquim |
Manager | Ms. LAI Mei Yee Josie |
Senior Technician | Dr. IP Tsz Ming Denis |
Technician | Mr. CHEUNG Chi Fai Randy |
Technician | Dr. KOK Tsz Wai Kevin |
Technician | Dr. LIN Yi Jyun Ivy |
Technician | Ms. SHAM Wai Yan Kathy |
Technician | Dr. SUEN Yick Keung |
Technician | Mr. YAU Chi Him Eric |
Technician | Ms. YEUNG Sai Ying Venus |
Research Assistant | Ms. CHEN Hui Chloe |
Enquiry on Core Laboratories, please contact us at Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 or 39434286
Biomechanics Core
1. Bio-mechanical Testers
a. Optics11 Life: Pavone Nanoindenter
b. Biomomentum Mechanical Tester Model: Mach-1 v500 csst
For SBS User:
Application for Access to SBS Core Laboratories for SBS Staffs and Students
https://corelab.sbs.cuhk.edu.hk/public
For Non-SBS User:
Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.
General Enquiry: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它
Dr. Kevin Kok | |
Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435754 |
Mr Randy Cheung | |
Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39438020 |
Dr. Denis Ip | |
Senior Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435722 |
Location: | LIBSB Room 512A |
Lab no.: | 39439432 |
Single Cell Omics Core
A major upgrade: Single Cell RNAseq v4 GEM-X solution
Research works using our services from SCOC can now be found inside the Publication thread below
Another option for scRNAseq: The single-cell Fixed RNA Profiling (scFRP)-Part I
Another option for scRNAseq: The single-cell Fixed RNA Profiling (scFRP)-Part II
Single Cell Gene Expression Low Throughput (LT) Solution service package
Spatial Omics literature database
Launch of Single Cell Fix RNA Profiling Solution
Application Unlocked: Single Cell Fixed RNA Profiling Solution on FFPE Samples
The SCOC provides a streamlined, cell-to-sequencing library service package for Single Cell RNA sequencing (scRNA-seq). It is capable to simultaneously profiling of transcriptomes ranging 500 – 10,000 cells in a single experiment. Different indexing systems are designed to label cell, transcript and sample origin in the sequencing library. This enables multiplexing of different sequencing libraries from multiple samples in the same Next Generation Sequencing (NGS) run. Libraries are Illumina-format compatible.
The service provided by SCOC includes an initial sample QC check, following by an evaluation of overall quality of sample for fitness to continue run. Single cells are then encapsulated in thousands of Gel Bead-in Emulsion (GEM) droplets, where cells are lysed and mRNA molecules reverse transcribed into cDNA. cDNA with specific cell barcodes are then pooled together and subjected to subsequent conventional sequencing library preparation. A final QC is performed on the final sequencing library to ensure sound quality for massively-parallel sequencing.
The SCOC welcomes interested PIs for experimental troubleshooting, intellectual advices as well as project discussions / collaborations.
Workflow overview
10X Chromium Next GEM Single Cell 3’ Gene Expression Workflow
Chromium Next GEM Chip:
RT, template switching
Pre-amp PCR
cDNA quality control
Sequencing library preparation
Sequencing library quality control
Please contact Dr Joaquim Vong (email: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 / 39430318) for service request, technical enquiry and project discussion.
For service request, please fill in our Sample submission form.
✓ Package set: From cell to sequencing library
✓ Cell quality and viability check / basic QC
✓ Sequencing library QC and quantification
✓ Intellectual advices (wet / dry lab)
✓ Troubleshooting
× Sequencing NOT included
× Repeated initial sample QC checks might incur extra charges
A. Sequencing library
B. QC reports for cDNA and sequencing library
C. Indexing information
The final product is a double-stranded cDNA molecule with sequencing adaptors and 3 different indices (10X barcode: cell info; UMI: transcript info; sample index: sample info). The sequencing library quality is evaluated by running into an Agilent 4200 TapeStation System.
Source: 10X Genomics
Users are required to fill in and sign the “Service Form for Single Cell Omics Core” prior to sample submission
https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400
All users MUST declare the nature of sample(s) submitted to SCOC FREE of any bio-hazardous material.
An initial assessment of cell quality will be conducted for the generation of data with optimal quality. Users may take the risk of sub-optimal data quality if the initial cell quality check fails.
Upon service booking, a copy of the confirmed service form will be forwarded to both the end user as well as the corresponding PI.
Initial sample submission evaluation (sample quality, amount, etc) is recommended to optimize sample quality, depending on the sample nature. Potential experimental discussion(s) might be arranged prior to samples submission.
The complete workflow take approximately 2 full working days. As only limited amount of samples can be performed per week, users are encouraged to optimize their sample quality before submission. Service bookings are arranged on a first-come-first-serve basis.
Once the service has started, service charge would be accounted for FULL service package price, as the reagents are on package stock basis. We are pledged to provide our service in a reasonable, efficient manner with quality.
10X Genomics – Single Cell Gene Expression
https://www.10xgenomics.com/products/single-cell-gene-expression
10X University – A step-by-step learning and training environment
https://www.10xgenomics.com/10x-university
10X Datasets
https://www.10xgenomics.com/resources/datasets
10X Publications
https://www.10xgenomics.com/resources/publications
Worthington Tissue Dissociation Guide
http://www.worthington-biochem.com/tissuedissociation
Dr Joaquim Vong | |
Scientific Officer | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39430318 |
Ms. Chloe Chen | |
Research Assistant | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435240 |
Location: | LIBSB Room 525 |
Lab no.: | 39434373 |
Genomics Core
For SBS User:
Application for Access to SBS Core Laboratories for SBS Staffs and Students
https://corelab.sbs.cuhk.edu.hk/public
For Non-SBS User:
Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.
General Enquiry: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它
Ms. Venus Yeung | |
Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39438157 |
Dr. Denis Ip | |
Senior Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435722 |
Location: | LIBSB Room 612 |
Lab no.: | 39436807 |
Common Research Core
1. Centrifugation
a. Hitachi CS-150GXII Micro Ultracentrifuge
b. Beckman Optima XPN-100 Ultracentrifuge
2. Chromatography System
a. BioRAD NGC Discover 10 Pro
3. Plate Reader
a. Thermo Varioskan LUX Multi-mode Microplate Reader
4. Imaging System
a. BioRad ChemDoc MP Imaging System
b. Cytiva ImageQuant 800 Imaging System (with true colour imaging)
5. Mass Spectrometry Systems
a. Agilent 6460 Triple Quad LC/MS
6. Real-Time PCRs
a. ABI QuanStudio 7 Flex Real-Time PCR System
b. ABI QuanStudio 7 Pro Real-Time PCR System
7. Digital PCR System
a. BioRAD Droplet Digital PCR System
For SBS User:
Application for Access to SBS Core Laboratories for SBS Staffs and Students
https://corelab.sbs.cuhk.edu.hk/public
For Non-SBS User:
Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.
General Enquiry: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它
Dr Suen Yick Keung | |
Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39439436 |
Dr. Denis Ip | |
Senior Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435722 |
Ms. Venus Yeung | |
Technician | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39438157 |
Location: | LIBSB Room 512 |
Lab no.: | 39439432 |