• RWD C200FL Automated Cell Counter with fluorescence (Cell viability check and counting)
• Light Microscope / Hemocytometer (Manuel cell counting)
• Compact cooling centrifuge
• 10X Controller (Single cell encapsulation)
• 10X Chromium iX Controller (Single cell encapsulation)
• 10X Visium CytAssist (Spatial profiling)
• PCR workstation
• PCRs, thermomixer, vortex, plate centrifuge
• Qubit 4 Fluorometer (DNA/RNA and library quantification)
• NanoString CosMx Spatial Molecular Imager (spatial multiomics single-cell profiling)
• 3DHistech TMA Master II (creating tissue microarray blocks)
• Boekel Scientific RapidFISH slide hybridization chamber

Please contact Dr Joaquim Vong (email: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 / 39430318) for service request, technical enquiry and project discussion.

For service request, please fill in our Sample submission form.

✓ Package set: From cell to sequencing library
✓ Cell quality and viability check / basic QC
✓ Sequencing library QC and quantification
✓ Intellectual advices (wet / dry lab)
✓ Troubleshooting

× Sequencing NOT included
× Repeated initial sample QC checks might incur extra charges

A. Sequencing library
B. QC reports for cDNA and sequencing library
C. Indexing information

The final product is a double-stranded cDNA molecule with sequencing adaptors and 3 different indices (10X barcode: cell info; UMI: transcript info; sample index: sample info). The sequencing library quality is evaluated by running into an Agilent 4200 TapeStation System.

Source: 10X Genomics

Users are required to fill in and sign the “Service Form for Single Cell & Spatial Omics Core” prior to sample submission
https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400

All users MUST declare the nature of sample(s) submitted to SCSOC FREE of any bio-hazardous material.

An initial assessment of cell quality will be conducted for the generation of data with optimal quality. Users may take the risk of sub-optimal data quality if the initial cell quality check fails.

Upon service booking, a copy of the confirmed service form will be forwarded to both the end user as well as the corresponding PI.

Initial sample submission evaluation (sample quality, amount, etc) is recommended to optimize sample quality, depending on the sample nature. Potential experimental discussion(s) might be arranged prior to samples submission.

The complete workflow take approximately 2 full working days. As only limited amount of samples can be performed per week, users are encouraged to optimize their sample quality before submission. Service bookings are arranged on a first-come-first-serve basis.

Once the service has started, service charge would be accounted for FULL service package price, as the reagents are on package stock basis. We are pledged to provide our service in a reasonable, efficient manner with quality.

1. Congratulations to the publication of the scRNAseq work by Prof. Ko Wing-Hung titled ‘Single-Cell RNA Sequencing Reveals Heterogeneity of ALI Model and Epithelial Cell Alterations after Exposure to Electronic Cigarette Aerosol’ on Heliyon (IF:3.4)
2. Congratulations to the publication of the scRNAseq work by Prof. Alfred Cheng titled ‘Targeting PPAR-gamma counteracts tumour adaptation to immune-checkpoint blockade in hepatocellular carcinoma’ on Gut (IF: 31.795)
3. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Targeting m6A reader YTHDF1 augments antitumour immunity and boosts anti-PD-1 efficacy in colorectal cancer’ on Gut (IF: 31.795)
4. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice’ on Cell (IF: 66.85)
5. Congratulations to the publication of the research work using our service by Prof. Sham Mai Har from SBS titled ‘Ptch1 is essential for cochlear marginal cell differentiation an stria vascular formation’ on Cell Reports (IF: 9.995)

10X Genomics – Single Cell Gene Expression
https://www.10xgenomics.com/products/single-cell-gene-expression

10X University – A step-by-step learning and training environment
https://www.10xgenomics.com/10x-university

10X Datasets
https://www.10xgenomics.com/resources/datasets

10X Publications
https://www.10xgenomics.com/resources/publications

Worthington Tissue Dissociation Guide
http://www.worthington-biochem.com/tissuedissociation