Single Cell & Spatial Omics Core
Official Launch of the Spatial Omics Services
A major upgrade: Single Cell RNAseq v4 GEM-X solution
Research works using our services from SCSOC can now be found inside the Publication thread below
Another option for scRNAseq: The single-cell Fixed RNA Profiling (scFRP)-Part I
Another option for scRNAseq: The single-cell Fixed RNA Profiling (scFRP)-Part II
Launch of Single Cell Fix RNA Profiling Solution
Application Unlocked: Single Cell Fixed RNA Profiling Solution on FFPE Samples
The SCSOC provides a streamlined, cell-to-sequencing library service package for Single Cell RNA sequencing (scRNA-seq). It is capable to simultaneously profiling of transcriptomes ranging 500 – 10,000 cells in a single experiment. Different indexing systems are designed to label cell, transcript and sample origin in the sequencing library. This enables multiplexing of different sequencing libraries from multiple samples in the same Next Generation Sequencing (NGS) run. Libraries are Illumina-format compatible.
The service provided by SCSOC includes an initial sample QC check, following by an evaluation of overall quality of sample for fitness to continue run. Single cells are then encapsulated in thousands of Gel Bead-in Emulsion (GEM) droplets, where cells are lysed and mRNA molecules reverse transcribed into cDNA. cDNA with specific cell barcodes are then pooled together and subjected to subsequent conventional sequencing library preparation. A final QC is performed on the final sequencing library to ensure sound quality for massively-parallel sequencing.
The SCSOC welcomes interested PIs for experimental troubleshooting, intellectual advices as well as project discussions / collaborations.
Workflow overview
10X Chromium Next GEM Single Cell 3’ Gene Expression Workflow
Chromium Next GEM Chip:
RT, template switching
Pre-amp PCR
cDNA quality control
Sequencing library preparation
Sequencing library quality control
Please contact Dr Joaquim Vong (email: Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 / 39430318) for service request, technical enquiry and project discussion.
For service request, please fill in our Sample submission form.
✓ Package set: From cell to sequencing library
✓ Cell quality and viability check / basic QC
✓ Sequencing library QC and quantification
✓ Intellectual advices (wet / dry lab)
✓ Troubleshooting
× Sequencing NOT included
× Repeated initial sample QC checks might incur extra charges
A. Sequencing library
B. QC reports for cDNA and sequencing library
C. Indexing information
The final product is a double-stranded cDNA molecule with sequencing adaptors and 3 different indices (10X barcode: cell info; UMI: transcript info; sample index: sample info). The sequencing library quality is evaluated by running into an Agilent 4200 TapeStation System.
Source: 10X Genomics
Users are required to fill in and sign the “Service Form for Single Cell & Spatial Omics Core” prior to sample submission
https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400
All users MUST declare the nature of sample(s) submitted to SCSOC FREE of any bio-hazardous material.
An initial assessment of cell quality will be conducted for the generation of data with optimal quality. Users may take the risk of sub-optimal data quality if the initial cell quality check fails.
Upon service booking, a copy of the confirmed service form will be forwarded to both the end user as well as the corresponding PI.
Initial sample submission evaluation (sample quality, amount, etc) is recommended to optimize sample quality, depending on the sample nature. Potential experimental discussion(s) might be arranged prior to samples submission.
The complete workflow take approximately 2 full working days. As only limited amount of samples can be performed per week, users are encouraged to optimize their sample quality before submission. Service bookings are arranged on a first-come-first-serve basis.
Once the service has started, service charge would be accounted for FULL service package price, as the reagents are on package stock basis. We are pledged to provide our service in a reasonable, efficient manner with quality.
10X Genomics – Single Cell Gene Expression
https://www.10xgenomics.com/products/single-cell-gene-expression
10X University – A step-by-step learning and training environment
https://www.10xgenomics.com/10x-university
10X Datasets
https://www.10xgenomics.com/resources/datasets
10X Publications
https://www.10xgenomics.com/resources/publications
Worthington Tissue Dissociation Guide
http://www.worthington-biochem.com/tissuedissociation
Dr Joaquim Vong | |
Scientific Officer | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39430318 |
Ms. Chloe Chen | |
Research Assistant | |
Email: | Email住址會使用灌水程式保護機制。你需要啟動Javascript才能觀看它 |
Tel no: | 39435240 |
Location: | LIBSB Room 525 |
Lab no.: | 39434373 |