B.Sc., M.Sc, Ph.D.
Room 620A, 6/F., Lo Kwee Seong Integrated Biomedical Sci. Bldg, Area 39, CUHK
Prof. ZHAO Hui (趙暉) is working at the School of Biomedical Sciences, The Chinese University of Hong Kong. He received his Bachelor Degree and Master Degree from Shandong University. He then went to Germany, and got his Ph.D. from the University of Essen, Germany. He had his post-doctoral training at the National Institutes of Health and Child Health and Development (NICHD) before he joined The Chinese University of Hong Kong in 2008. Professor Zhao’s research interests cover developmental biology and cancer biology. His laboratory studies the mechanism of neural crest differentiation, germ layer formation and cell migration, and how these multiple events affect the embryonic patterning. In the past few years, he also studied the tumorigenesis of neuroblastoma. Recently his group utilized TALEN and Cas9 nucleases to do gene targeting in Xenopus, zebrafish, and stem cells. He has published over 70 papers in high impact journals including PNAS, Development, EMBO Journal, Nucleic Acids Research and Journal of Biological Chemistry. He serves as reviewers for various magazines including PNAS, Development, and Plos Biology. His research is supported by the funds from the Ministry of Science and Technology, the National Natural Science Foundation of China and Hong Kong Research Grants Council.
- Genetic and epigenetic regulation in the neural crest formation.
- Molecular mechanisms of germ layer formation during early embryonic development.
- Gene regulation and functional genomics in neuroblastoma.
- Genome editing in Xenopus embryos and stem cells.
- Molecular mechanisms of tissue regeneration and repair.
- Li TF, Deng Y, Shi Y, Tian RJ, Chen YL, Zou L, Kazi JU, Rönnstrand L, Feng B, Chan SO, Chan WY, Sun J, and Zhao H. (2018). Bruton’s tyrosine kinase potentiates ALK signaling and serves as a potential therapeutic target of neuroblastoma. Oncogene In press.
- Mao CZ, Zheng L, Zhou YM, Wu HY, Xia JB, Liang CQ, Guo XF, Peng WT, Zhao H, Cai WB, Kim SK, Park KS, Cai DQ, and Qi XF. (2018). CRISPR/Cas9-mediated efficient and precise targeted integration of donor DNA harboring double cleavage sites in Xenopus tropicalis. FASEB J. [Epub ahead of print].
- He XY, Tan ZL, Mou Q, Liu FJ, Liu S, Yu CW, Zhu J, Lv LY, Zhang J, Wang S, Bao L, Peng B, Zhao H, and Zou L. (2017). microRNA-221 enhances MYCN via targeting nemo-like kinase, and functions as an oncogene related to poor prognosis in neuroblastoma. Clin Cancer Res. 23: 2905-2918.
- Liu Z, Guo J, Wang Y, Weng Z, Huang B, Yu MK, Zhang X, Yuan P, Zhao H, Chan WY, Jiang X, Chan HC. (2017). CFTR-β-catenin interaction regulates mouse embryonic stem cell differentiation and embryonic development. Cell Death Differ. 24: 98-110.
- He, X., Tan, C., Wang, F., Wang, Y., Zhou, R., Cui, D., You, W., Zhao, H., Ren, J., Feng, B. (2016). Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res. (2016) 44(9): e85.
- Wang, C. D., Kam, R. T. K., Shi, W. L., Xia, Y., Chen, X. F., Cao, Y., Sun, J., Du, Y., Lu, G., Chen, Z. J., Chan, W. Y., Chan, S. O., Deng, Y., and Zhao, H. (2015). The proto-oncogene transcription factor Ets1 regulates neural crest development through Histone Deacetylase 1 to mediate output of bone morphogenetic protein signaling. J Biol Chem, 290(36), 21925-21938.
- Shi, Z., Wang, F., Cui, Y., Liu, Z., Guo, X., Zhang, Y., Deng, Y., Zhao, H., and Chen, Y. (2015). Heritable CRISPR/Cas9-mediated targeted integration in Xenopus tropicalis. FASEB J, 29(12), 4914-4923.
- Shi, W. L., Xu, G., Wang, C. D., Sperber, S. M., Chen, Y. L., Zhou, Q., Deng, Y., and Zhao, H. (2015). Heat shock 70kDa protein 5 (Hspa5) is essential for pronephros formation by mediating retinoic acid signaling. J Biol Chem, 290(1), 577-589.
- Liu, Y., Luo, D. Y., Lei, Y., Hu, W., Zhao, H., and Cheng, C. H. K. (2014). A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish. Methods, 69(1), 58-66, S1046-2023.
- Hu, J., Lei, Y., Wong, W. K., Liu, S., Lee, K. C., He, X., You, W., Zhou, R., Guo, J. T., Chen, X., Peng, X., Sun, H., Huang, H., Zhao, H., Feng, B. (2014). Direct activation of human and mouse Oct4 genes using engineered TALE and Cas9 transcription factors. Nucleic Acids Res., 42(7), 4375-4390.
- Guo, X. G., Zhang, T. J., Hu, Z., Zhang, Y. Q., Shi, Z. Y., Wang, Q. H., Cui, Y., Wang, F. Q., Zhao, H. and Chen, Y. L. (2014). Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis. Development, 141, 1-8.
- Kam, R. K. T., Shi, W., Chan, S. O., Chen, Y., Xu, G., Lau, C. B. S., Fung, K. P., Chan, W. Y., Zhao, H. (2013). dhrs3 attenuates the retinoic acid signaling and is required for early embryonic patterning. J Biol Chem, 288(44), 31477-31487.
- Lei, Y., Guo, X. G., Liu, Y., Cao, Y., Deng, Y., Chen, X. F., Cheng, H. K. C., Dawid, I. B., Chen, Y. L. and Zhao, H. (2012). Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs). Proc Natl Acad Sci USA, 109(43), 17484-17489.
- Zhao, H., Han, D., Pieler, T. and Chen, Y. (2012). Hhex induced conversion of intestinal to ventral pancreatic precursor cells results in the formation of giant pancreata in Xenopus embryos. Proc Natl Acad Sci USA, 109, 8594-8599.
- Kam, K. T., Deng, Y., Chen, Y. L., and Zhao, H. (2012). Retinoic acid synthesis and functions in early embryonic development. Cell and BioSci, 2, 11.
- Wang, C. D., Liu, Y., Chan, W. Y., Chan, S. O., Grunz, H. and Zhao, H. (2011). Characterization of three synuclein genes in Xenopus laevis. Dev. Dyn., 240(8), 2028-2033.
- Tanegashima, K., Zhao, H., Rebbert, M. and Dawid, I. B. (2009). Notochord differentiation requires activation of the unfolded protein response. Development, 136, 3543-3548.
- Zhao, H., Tanegashima, K., Ro, H. and Dawid, I. (2008). Lrig3 regulates neural crest formation in Xenopus by modulating Fgf and Wnt signaling pathways. Development, 135(7), 1283-1293.
- Tanegashima, K., Zhao, H. and Dawid, I. (2008). WGEF activates Rho in the Wnt-PCP pathway and controls convergent extension in Xenopus gastrulation. EMBO. J., 27(4), 606-617.
- Zhao, H., Cao, Y. and Grunz, H. (2003). XXBP-1, a leucine zipper transcription factor, is involved in the BMP-4 signaling pathway. Dev Biol, 257, 278-291.