School of Biomedical Sciences
生物醫學學院
The Chinese University of Hong Kong 香港中文大學

Histology Core

Histology Core

About us

Equipment

1. Microscopes
       a. ZEISS AxioLab 5 upright microscope

2. Tissue Processors
       a. Thermo Excelsior ES
       b. Thermo Excelsior AS

3. Tissue Embedding Centres
       a. Histostar Embedding Centre

4. Cryotomes and Microtomes
       a. Epredia NX70 Cryostat
       b. Leica CM3050S + CyroJane Tape Transfer System
       c. Leica CM1950 with UV disinfection and vacutome
       d. Compresstome VF-700-OZ
       e. Leica RM2235 Rotary Microtome with cooling head

5. Staining Facilities
       a. Thermo PT module Antigen Retriever
       b. Staining stations (AirClean System 48" wide ductless chemical workstation)
       c. MEDITE TST 44 Automatic Slide Staining Station
       d. Epredia CTM6 Coverslipper

General Information

For SBS User:

Application for Access to SBS Core Laboratories for SBS Staffs and Students

https://corelab.sbs.cuhk.edu.hk/public

For Non-SBS User:

Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to This email address is being protected from spambots. You need JavaScript enabled to view it. .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.

General Enquiry: This email address is being protected from spambots. You need JavaScript enabled to view it.

Contacts

Mr Randy Cheung  
Technician  
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel no: 39438020
   
Ms. Kathy Sham  
Technician  
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel no: 39435668
   
Location: LIBSB Room 212
Lab no.: 39435751

Staining system in bench top hood

Staining

Features: - A series of organic solvent in tanks which include xylene, absolute ethanol, 95% ethanol, 80% ethanol, 70% ethanol are set inside the bench top hood for de-waxing, dehydrating paraffin sections;

- The series also include the common histological stain - Haematoxylin and Eosin (H&E) for routine morphological staining of sections;

- provide special stains on request.

Staff-in-charge:

- Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212

 

 

PT module (Thermo) for Antigen Retrieval

PT module Thermo for Antigen Retrieval
The module offers consistent antigen retrieval for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections using gentle heat.
Features:

-one-touch operation via built-in touchscreen  

-Programmed time and temperature setting

-2 reaction baths, each with 24-slide capacity,
for simultaneous operation of two independent procedures

Staff-in-charge: - Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212

Digital Imaging System
Q-Imaging Color CCD Digital Camera & Carl Zeiss Microscope Axiophot 2

Pathcentre (Shandon)

Q Imaging

Features:

This system includes:

1. Zeiss Axiophot-2 microscope

- Objectives include 2.5x, 5x, 10x, 20x, 40x and 63x, 100x oil lens

- Transmitted light source and 100W fluorescence light source from HBO lamp

- 3 fluorescence filters with excitation wavelengths at 420nm, 520nm and 590nm

- fitted with Phase contrast, DIC and polarizer

2. Image capture and analysis software

- Q-Capture Pro 7 & Metamorph v 7 software

Staff-in-charge:

- Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212B

 

Spot Digital Camera & Leica Microscope Biological Imaging System

spot

Features: - This system includes the Leica DM LA upright microscope with motorized stage and removable specimen holder

- 5x, 10x, 20x, 40x and 100x objectives

- Spot imaging software is fitted for image capture and analysis

Staff-in-charge:

- Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212

Tissue Processor

Pathcentre (Shandon)

Pathcentre

Tissue processor – Thermo Excelsior ES

Tissue processor

Features:

- Shandon Pathcentre and Thermo Excelsior ES are totally enclosed tissue processor that comprise a command console and a processing module

- The command Console can store 9 programs of up to 16 steps which include 10 processing steps, 4 wax stages and 2 flush cycles

- Tissues are processed by selecting program with temperature, vacuum/pressure, agitation, time parameters setting

- Processing module can each process up to 324 cassettes;

Staff-in-charge:

- Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212

 

Histo-Embedding Centre + cold plate

Thermo Shandon Histocentre 3
Thermo Shandon

 

 

Embedding Centre – Leica EG 1150

Embedding Centre

Features: - provides all the components necessary to effectively perform histological paraffin embedding

- LCD touch screen
  Tissue storage tank
  Paraffin dispenser
  Hot spot with light
  Cold spot and cold plate;

Staff-in-charge:

- Corinna
Back-up Support: - Ming/Anny/Samuel
Location: LIBSB 212