School of Biomedical Sciences
生物醫學學院
The Chinese University of Hong Kong 香港中文大學

Macromolecular and Microarray Core

Bio-Rad ChemiDoc Imaging System (MMCore 15a)

ChemiDoc Imaging System
Features:

- For Chemiluminscence detection (ECL Western Blot Imaging)

- Enable Colormetic protein detection (Coomassie blue or stain free gel) 

- Identify and quantify proteins with chemiluminescence,or colorimetric detection

- Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging 

- Automatic focus and exposure, with preview features to ensure optimal images 

- Automatic selection of optimal light source by application 

- Equipped with BioRad Image Lab software for further data analysis 

- Data image files save as TIFF or JPEG format

- No X-ray film required

Staff-in-charge:

 - Ms. Venus Yeung
Back-up Support: -Mr. Randy Cheung / Mr. Eric Yau
Location: LIBSB 612

 

Bio-Rad ChemiDoc MP Imaging System (MMCore 15b)

MMC15b ChemiDoc MP
Features:

- Fluorescence dectection (protein gel or western blot)

- Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging 

- Automatic focus and exposure, and preview features ensure optimal images. 

- Automatic selection of optimal light source by application, 

- Equipped with BioRad Image Lab software for further data analysis (free of charge)

- Data image files save as TIFF or JPEG format

- Equipped with different filters for various fluorescence protein detection:
                  (a) 518–546 nm filter for blue-excitable fluorophores and stains

                  (b) 700–730 nm filter for far red-excitable fluorophores and stains

                  (c) 813–860 nm for near IR-excitable fluorophores and stains

Staff-in-charge:

 - Ms. Venus Yeung
Back-up Support: -Mr. Randy Cheung / Mr. Eric Yau
Location: LIBSB 612

 

SpectraMax® i3x Multi-Mode Microplate Detection Platform with Automatic Injector Cartridge

Molecular2
Features:

- Flexible monochromator-based multimode plate reader;

- Equipped with 2x Automatic Injector Cartridge for precise measurement

- Allows rapid and accurate measurement of luminescent or fluorescent signal.

- Reverse Prime Function for reagent to minimize dead volume

- The Injector Cartridge enables inject-and-read measurement

- 6, 12, 24, 96 & 384 well format;

- Wavelength Range:

    1. Absorbance: 230-1000 nm 
    2. Fluorescence: 250-840 nm 
    3. Luminescence: 300-850 nm ;

- Dual Luciferase Reporter assay

- ATP assay system

- Spectrum and well scanning;

- Endpoint and kinetic reading;

- Temperature control and shaking;

- Filter Cartridges: 
    1. Fluorescence Polarization FP (fluorescein & Rodamine) 
    2. Cisbo® HTRF.

Staff-in-charge:

 - Ms. Venus Yeung
Back-up Support: -Mr. Randy Cheung / Mr. Eric Yau
Location: LIBSB 612

Hitachi CS 150GXII Micro Ultracentrifuge

150GXII
Features: Temperature Range: 0 – 40 ℃

Available Rotors:

- S140-AT Fixed angle (35°) Titanium Rotor, 10 × 2mL,
max 140,000rpm / 1,048,000 x g, K factor: 5

- S52-ST Swinging-Bucket (180°) Rotor , 4 × 5mL,
max 52,000rpm / 275,458 x g, K factor: 78.6

Staff-in-charge:

 - Venus
Back-up Support: - Randy / Eric / YK Suen
Location: LIBSB 612

 

Beckman Optima XPN 100 ultracentrifuge

 xpn100
Features: Available Rotors:

- Type 45 Ti Rotor, Fixed Angle, Titanium, 6 x 94 mL, 45,000 rpm,
235,000 x g

- Type 70 Ti Rotor, Fixed Angle, Titanium, 8 x 39 mL, 70,000 rpm,
504,000 x g

- Type 90 Ti Rotor, Fixed Angle, 8 x 13.5 mL, 90,000 rpm, 694,000 x g

- Type 100 Ti Rotor, Fixed Angle, 8 x 6.8 mL, 100,000 rpm, 802,400 x g

- NVT 65.2 Rotor Pkg, Near-Vertical, 16x5.1 mL, 65,000 rpm, 416,000 x g

- SW 41 Ti Rotor, Swinging Bucket, 6 x 13.2 mL, 41,000 rpm, 288,000 x g

- Refrigeration

- Please use compatible centrifuge tubes.

Staff-in-charge:

 - Venus
Back-up Support: - Randy / Eric / YK Suen
Location: LIBSB 612

 

Beckman Avanti J-E High Speed centrifuge

high
Features: Available Rotors:

- JA-25.50 Rotor, Single Lock Lid, 8 x 50 mL, 25,000 rpm, 75,600 x g

- JA-20.1 Rotor, Biosafety Lid, 32 x 15 mL, 20,000 rpm, 51,500 x g

- JA-20 Rotor, Fixed Angle, Aluminum, Biosafety Lid, 8 x 50 mL, 20,000 rpm, 48,400 x g

- JS-13.1 Rotor & Handle, Swinging-Bucket, 6x50mL, 13,000rpm, 26,500 x g

- J-LITE JLA-16.250 Rotor, Single Lock, 6x250mL, 16,000 rpm, 38,400 x g

- J-LITE JLA-10.500 Rotor, Biosafety, 6x500 mL, 10,000 rpm, 18,600 x g (only 4 Aluminum, Biosafety Liquid Canisters)

- Refrigeration

- Please use compatible centrifuge tubes.

Staff-in-charge:

 - Venus
Back-up Support: - Randy / Eric / YK Suen
Location: LIBSB 612

 

Beckman J6-MI Low Speed High Capacity Centrifuge

 low speed
Features: - Adaptors for 15 mL x 28, 50 mL x 8, 250 mL x 4, 500mL x 4 and 2 plates

- Max Speed: 4,200 rpm

- Refrigeration

- Please use compatible centrifuge tubes.

Staff-in-charge:

 - Venus
Back-up Support: - Randy / Eric / YK Suen
Location: LIBSB 612

 

Eppendorf 5810R centrifuge

5810R
Features: - Adaptors for 15 mL, 50mL and plates

- Max Speed: 4,000 rpm

- Refrigeration

Staff-in-charge:

 - Venus
Back-up Support: - Randy / Eric / YK Suen
Location: LIBSB 612

 

 

ABI ViiA 7 Real Time PCR System

viia7
Features: - Adapt to 96-well (fast) 0.1mL plate, 384-well plate and 8-well strip (0.1 mL) with optical flat caps

- Calibrated dyes: SYBR Green Dye I, FAM, VIC, NED, ROX, TAMRA, MELTDOCTOR

- Light source: OptiFlex System halogen lamp

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

ABI StepOne Real-Time PCR System

 step one
Features: - Adapt to 48-well plate and 8-well strip (0.1 mL) with optical flat caps

- Calibrated dye: FAM, SYBR Green, VIC, JOE, ROX

- Light source: Sensitive LED-Based 3-Color Fluorescence Reading

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

ABI 7900HT Fast Real Time PCR

7900
Features: - Adapt to 96-well (fast) 0.1mL plate and 384-well plate

- Calibrated Dyes: SYBR Green Dye I, FAM, VIC, JOE, NED,ROX, TAMRA, TET

- Light source: Laser 488 nm argon-ion excitation source

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

ABI QuantStudio 7 (QS7) Flex Real Time PCR System

 QS7
Features: - Suitable for 384-well (Standard/ Fast) 0.1mL Optical Real Time PCR plate,

- Calibrated dyes: SYBR Green Dye I, FAM, VIC, NED, ROX, TaqMan Probes

- Application: Gene Expression, SNP Genotyping Assay, Protein Thermal Shift

- Light source: OptiFlex System halogen lamp

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

Bio-Rad QX200 Droplet Digital PCR System

QX200
Features: - System work with QX 200 droplet generator & digital PCR reader

- Suitable for 96-well plate (0.1mL Optical Bio-Rad Real Time PCR plate)

- Assay dye : digital PCR (ddPCR) Supermix for Probes, digital PCR (ddPCR) EvaGreen Supermix

- Application: Copy Number Variation

                    Rare mutation and sequence detection

                    Gene Expression (low gene expression)

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612
Booking: No online booking available

Please contact Venus Yeung / Randy for booking

Affymetrix Microarray System

photo 5 1
Features: - Equipped with Hybridization Oven 640, Fluidics Station 450 and GeneChip Scanner 3000 7G system;

- Incorporate advanced gene expression analysis and genotyping studies;

- Transcription detection e.g. coding mRNA, Copy number variation (CNV), Single nucleotide polymorphism (SNP);
- Genechips and consumables are not provided by Core Labs.

Staff-in-charge:

 - YK Suen / Eric
Location: LIBSB 612

 

Partek Genomic Suite and Partek Pathway Software for Microarray data analysis

 
Features: - Partek Genomics Suite is a comprehensive suite of advanced statistics and interactive data visualization, specifically designed to reliably extract biological signals from noisy data.

- The software is unique in supporting all microarray.

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

Agilent 2100 Bioanalyzer

bioana
Features: - Serves as a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells Serves as a review control taken during the target preparation prior to committing to the hybridization of the Microarray

- Genechips and consumables are not provided by Core Labs.

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric/YK Suen
Location: LIBSB 612

 

Automated Next Generation Sequencing (NGS) System: The Ion Chef System & Ion Torrent S5 Sequencer


The Ion Chef System Ion Torrent S5 Sequencer
The Ion Chef System and the Ion S5 Sequencing System enable targeted next-generation sequencing workflow for panels, microbes, exomes, FFPE, transcriptomes and copy number analysis.
Features:

Ion Chef System

  •  The system helps to simply the next-generation sequencing workflow for Ion S5,
     
  •  It incorporates automated Ion Ampliseq library preparation, template preparation and chip loading in the system with the use of pre-packaged cartridge-based reagent kits for library preparation.
  • It enables reproducible template preparation on 2 chips for multiple samples for sequencing within hours.
  •  Ion chips (Ion 510, 520, 530 & 540 chips are compatible for use) and consumables required will not be provided by Core Labs.


Ion S5 Sequencing System

- This system has the capability to multiplex thousands of targets and hundreds of samples in a single sequencing run.

-  DNA/RNA for sequencing can be as low as 1-10ng using Ion AmpliSeq technology.


- Workflow for panels, microbes, exomes and transcriptomes on Ion S5 System:

  Ion 510 Chip Ion 520 Chip Ion 530 Chip Ion 540 Chip
Reads   2-3 million 4-6 million 15-20 million 60-80 million
Output 200 bp 0.3-0.5 Gb 0.6-1 Gb 3-4 Gb 10-15 Gb
400 bp 0.6-1 Gb 1.2-2 Gb 6-8Gb —
Run times 200 bp 2.5 hr 2.5 hr 2.5 hr 2.5 hr
400 bp 4 hr 4 hr >4 hr —

Analysis time

 200 bp 2 hr 5 hr 8 hr 16.5 hr
400 bp 6.5 hr 8 hr 17.5 hr —

-Workflow for long-read sequencing for research applications such as human leukocyte antigen (HLA) typing or metagenomic analysis on Ion S5 System

Ion 510 Chip Ion 520 Chip Ion 530 Chip Ion 540 Chip
Reads 600bp —  3-4 million 9-12 million —
Outputs* 600bp —  0.5-1.5 Gb  1.5-4.5 Gb —
Run time 600bp — 4hrs 4hrs —
Analysis time†† 600bp — 8hrs 17hrs —

 

Built-in informatics

  • Data generated will automatically be uploaded from Torrent Suite Software to Ion Reporter™ Software for further analysis.

Staff-in-charge:

 - Venus
Back-up Support: - Randy/Eric
Location: LIBSB 612
Booking of use:
  • For SBS MEMBERS ONLY
  • Training is needed for all uses. Please contact Venus in person for discussion and booking.
  • Booking system will be on daily basis, from Monday to Friday ONLY.