Flow Cytometry Core
1. Flow Cytometry
a. BD FACSDiscover S8 Cell Sorter with BD CellView Image Technology with Class 2A Biosafety Cabinet
b. BD Symphony A5.2 SORP High-parameter Cell Analyzer
c. BD Aria Fusion Cell Sorter and Cell Analyzer
d. FlowJo Software (Plugins: FlowSOM, UMAP, FitSNE etc)
2. Metabolimetry
a. Seahorse XFe96 Extracellular Flux Cell analyzer
3. Cryogenic Grinding
a. SPEX 6775 Freezer/Mill Cryogenic grinder
4. Imaging
a. Thermo Fisher EVOS M5000 Imaging System
For SBS User:
Application for Access to SBS Core Laboratories for SBS Staffs and Students
https://corelab.sbs.cuhk.edu.hk/public
For Non-SBS User:
Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to This email address is being protected from spambots. You need JavaScript enabled to view it. .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.
General Enquiry: This email address is being protected from spambots. You need JavaScript enabled to view it.
Mr Eric Yau | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39434286 |
Ms. Kathy Sham | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39435668 |
Ms. Venus Yeung | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39438157 |
Dr Suen Yick Keung | |
Technician | |
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Tel no: | 39439436 |
Location: | LIBSB Room 412 |
Lab no.: | 39438022 |
NEON Transfection System
Features: |
- Working in Class II Biosafety Cabinet is recommended - Reagent kits are NOT included - CO2 incubator |
Staff-in-charge: |
- Eric |
Back-up Support: | - Venus / Randy |
Location: | LIBSB 412B |
Features: | - Automated single-cell isolation & preparation system
- 96 single-cell for downstream analysis - Capture -> Wash -> Lyse -> RT -> Preamp -> Harvest - Integrated Fluidic Circuit (IFC) chip: 1. Small cell: 5–10 µM 2. Medium Cell: 10 -17 µM 3. Large Cell: 17-25 µM - IFC chips and other necessary consumables are not provided |
Staff-in-charge: |
- Eric |
Back-up Support: | - Venus / YK Suen |
Location: | LIBSB 412A |
Features: |
-is a high-throughput real-time PCR system and a downstream equipment for C1 single cell prep system -enables real-time quantitative PCR (qPCR), SNP genotyping, gene expression profiling and digital PCR assays -uses the Integrated Fluidic Circuit (IFC) technology fluidics chip (96x96 or 48x48). - operates in either end-point or real-time detection mode. |
Staff-in-charge: |
- Eric |
Back-up Support: | - Venus /Randy |
Location: | LIBSB 412B |
Seahorse XFe96 Extracellular Flux Analyzer
Features: |
- Studies two major energy producing pathways of the cell – mitochondrial respiration and glycolysis simultaneously in a 96 -well microplate format in real-time and temperature controlled environment - Measures simultaneously oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in all wells, or please refer to Seahorse website for other applications at
- Detects cellular responses to up to 4 substrates, inhibitors, or other drugs in an automated compounds mixing and measuring - Optimizing conditions is recommended. - Reagent kits and consumables are NOT included. - CO2 & non-CO2 incubator, water bath and BSC are included. Users are responsible for preparing the medium and reagents, performing cell washing, and loading the assay cartridges. |
Staff-in-charge: |
- Eric |
Back-up Support: | - Venus / Randy |
Location: | LIBSB 412B |
Features: |
The system is equipped with an inverted fluorescence microscope, Perfect Focus System (PFS), and an incubation chamber for time lapse live cell experiments
- Light source: tungsten lamp & metal halide; - Fluorescence filter cube: DAPI, FITC, GFP, TRITC and Cy5 - Objectives: 10x, 20x and 40x Phase objectives; 20x DIC, 40x DIC (oil) and 60x DIC (oil); - Software: Nikon Elements BR. |
Staff-in-charge: |
- Eric |
Back-up Support: | - Venus / YK Suen |
Location: | LIBSB 412 |
Seahorse XFe96 Extracellular Flux Analyzer