- 96 single-cell for downstream analysis

- Capture -> Wash -> Lyse -> RT -> Preamp -> Harvest

- Integrated Fluidic Circuit (IFC) chip: 1. Small cell: 5–10 µM 2. Medium Cell: 10 -17 µM 3. Large Cell: 17-25 µM

- IFC chips and other necessary consumables are not provided

Staff-in-charge:

-is a high-throughput real-time PCR system and a downstream equipment for C1 single cell prep system

-enables real-time quantitative PCR (qPCR), SNP genotyping, gene expression profiling and digital PCR assays 

-uses the Integrated Fluidic Circuit (IFC) technology fluidics chip (96x96 or 48x48). 

- operates in either end-point or real-time detection mode.

Staff-in-charge:

- Studies two major energy producing pathways of the cell

– mitochondrial respiration and glycolysis simultaneously in a 96

-well microplate format in real-time and temperature controlled environment 

- Measures simultaneously oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in all wells, or please refer to Seahorse website for other applications at

http://www.agilent.com/en-us/products/cell-analysis-(seahorse)/how-seahorse-xf-analyzers-work

- Detects cellular responses to up to 4 substrates, inhibitors, or other drugs in an automated compounds mixing and measuring 

- Optimizing conditions is recommended. 

- Reagent kits and consumables are NOT included.

- CO2 & non-CO2 incubator, water bath and BSC are included. Users are responsible for preparing the medium and reagents, performing cell washing, and loading the assay cartridges.

Staff-in-charge:

The system is equipped with an inverted fluorescence microscope, Perfect Focus System (PFS), and an incubation chamber for time lapse live cell experiments

- Camera: Nikon DS-Qi2 Monochrome

- Light source: tungsten lamp & metal halide;

- Fluorescence filter cube: DAPI, FITC, GFP, TRITC and Cy5

- Objectives: 10x, 20x and 40x Phase objectives; 20x DIC, 40x DIC (oil) and 60x DIC (oil);

- Software: Nikon Elements BR.

Staff-in-charge: