Microscopy & Imaging Core
1. Microscopes
a. Scientifica Hyperscope Multi-photon System
b. Leica TCS SP8 WLL Inverted Confocal Microscope with Tokai-hit Incubation System
c. Olympus FV1200 Inverted Confocal Microscope with Ibidi Incubation System
d. Molecular Device ImageXpress Micro Confocal High-Content Imaging System
e. SciMedia MiCAM03 High Speed Imaging System
f. Nikon Ti-2 Inverted Fluorescence Microscope with Motorized stage and Okolab Incubation System
g. Olympus IX83 Inverted Fluorescence Microscope with Aurox Clarity Laser Free Confocal System
h. Nikon Ni-U Eclipse Upright Microscope with fluorescence attachment
i. ZEISS Axioscan 7 Microscope Slide Scanner
j. LiTone XL Light-sheet Microscope System
k. Abberior FACILITY LINE – STED Super-resolution Nanoscope (coming soon)
l. Imaris 10 Imaging software
2. Electron Microscopes
a. Hitachi HT7700 Transmission Electron Microscope
b. Hitachi SU8010 Cold Field Scanning Electron Microscope
c. Auxiliary instruments:
i. Tousimis PVT30 Samdri Critical Point Dryer
ii. Quorum Q150TES Sputter Coater
iii. Leica UCT7 Ultratome
iv. RMC Glass Knife Maker (GKM2)
For SBS User:
Application for Access to SBS Core Laboratories for SBS Staffs and Students
https://corelab.sbs.cuhk.edu.hk/public
For Non-SBS User:
Access of facilities to non-SBS users may be considered. Principal investigator of the project please submits the request to This email address is being protected from spambots. You need JavaScript enabled to view it. .The principal investigator, together with the end-users, will be invited for a discussion on project requirements and facility usage procedures in person. Requests of non-PIs will not be entertained.
General Enquiry: This email address is being protected from spambots. You need JavaScript enabled to view it.
Ms Josie Lai | |
Senior Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39436804 |
Dr. Kevin Kok | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39435754 |
Dr. Ivy Lin | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39430572 |
Location: | LIBSB Room 108-110, 115 |
Lab no.: | 39436843 |
µCT 35 is used to examine diverse materials such as ex-vivo bone samples and medical implants and are calibrated for measurement of biological tissue densities, and powerful 3D analysis routines including distance transformation, allowing measurement of thickness and separation of features like trabecular bone structure and blood vessels. The sample changer accommodates 8 sample holders. Depending on scanner type and sample holder diameter, scan position and protocol are specified for all samples interactively during loading, after which scans are performed unattended in batch mode. It also provides a software suite that offers a comprehensive solution for scanning, 3D analysis, visualization, image management and data import/export. | |
Specifications | µCT 35 |
Type | Desktop cone-beam micro-CT
Fully shielded No external shielding required |
X-ray source | Sealed
Air-cooled Maintenance-free 30 - 70 kVp / 20 - 50 keV (160 µA) |
Detector | 2048 x 256 elements, 24 µm pitch |
Resolution | ‹ 5 µm 10% MTF
1.75 - 72 µm nominal isotropic (pixel size) |
Image matrix | 512 x 512 to 4096 x 4096 pix |
Max. scan size | 37.9 x 120 mm (ØxL) |
Max. specimen size | 75.8* x 140 mm (ØxL) [ *Density calibration valid up to 36.9mm] |
Applications | Trabecular bone evaluation:
• Segmentation of bone • Trabecular thickness • Trabecular separation • Finite element analysis of trabecular stress
Material Applications (µCT 35): • Scaffold thickness map • Freeze-dried sample |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 115 |
Booking | For SBS MEMBERS ONLY and not open to non-SBS users. |
Micro-CT User Guidelines |
Features: |
White confocal combines a white light laser source, tunable excitation filtering, tunable beam-splitting and tunable emission-detection – with full access to the keys for all colors. It allows users to specify any excitation wavelength between 470nm and 670nm. Together with an AOBS (acoustical optical beam splitter) up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. Because the WLL system is a pulsed laser it can be used in conjunction with hybrid detectors to provide gated suppression (removal) of auto-fluorescence and reflected light. The SP8's spectral detection system uses a prism spectrometer based emission separation (not filters) that allows user selection of emission detection bandwidth as well as spectral scanning of emission profiles. This allows users to precisely select any emission band-pass they require. Additionally, the system can scan at 8000Hz for ultra-fast scanning (and greatly reduced photo-bleaching) via its selectable resonant scanner. It is fully enclosed for heating and environmental control with media perfusion capabilities and CO2 control. The system is ideal for imaging live cells, tissue in culture dishes and chambered cover glass (#1.5). Microscope
An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light. An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.
Reflection/fluorescence mode (spectral detection range 400-800 nm)
|
Staff-in-charge: |
Anny Cheung |
Back-up Support: | Josie Lai |
Location: | LIBSB 108A |
Remarks: |
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. |
Features: | - Equipped for fixed and live cell imaging, time-lapse imaging, multi-fluorescence z-stack 3-D, FRET/FRAP, SIM/HSD, multi-position time-lapse and mosaic outline imaging;
|
||||||||||||||||||
Staff-in-charge: |
- Josie | ||||||||||||||||||
Back-up Support: | - Anny/Samuel | ||||||||||||||||||
Location: | LIBSB 108B | ||||||||||||||||||
Remarks: | For SBS Experienced and Trained user only.
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. - Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
|
|
Features: | - Equipped for fixed and live cell imaging, time-lapse imaging and multi-fluorescence z-stack 3-D imaging;
- Connected to an IX81 inverted microscope with objectives 4x, 10x, 20x, 40x, 63x oil and epi-fluorescence filters for DAPI, FITC and TRITC; - Incubation chamber for live cell imaging; - Laser sets of spectral type multi-Argon (458, 488, 515) LD405, LD559 and LD635. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 108B |
Remarks: | For SBS Experienced and Trained user only.
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. - Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
Features: | - Equipped for fixed/live cell imaging; time-lapse imaging and high-speed multi-fluorescence optical sectioning;
- Connected to Leica DMI6000 inverted microscope with fluorescence filters for I3 (Ex 450-490 / Em LP515) and N2.1 (Ex 515-560 / Em LP590); - Laser sets multi-Argon 458, 476, 488, 514, DPSS 561 and HeNe633; - Objectives: 10x, 20x, 40x, 40x oil, 63x water, 100x oil. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Remarks: | - Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice.
- Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
Features: |
- Equipped for fixed and live cell imaging
Microscope • UPLSAPO4X, N.A. 0.16, WD 13 Filter sets: DAPI/Hoechst, FITC/EGFP, PI/TRITC, Cy5 Light source: X-Cite 120Q Metal halide or CoolLED pE4000 UV with 16 Selectable wavelengths including 365, 385, 405, 435, 460, 470, 490, 500, 525, 550, 580, 595, 635, 660, 740, 770nm Software interface: CellSens Dimension Camera: DP80 Double-CCD
Monochrome CCD: |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Features: | Microscope
- Zoom range: - Objectives: Filter sets: DAPI, GFP, Cy3, Cy5 Software interface: CellSens Camera: DP80 Double-CCD Color CCD: Resolution at 12.5 megapixels (4080 x 3072) Monochrome CCD: |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Features: |
Imaris is Bitplane's core scientific software module that delivers all the necessary functionality for data visualization, segmentation, analysis and interpretation of 3D and 4D microscopy datasets.
Modules available:
|
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny/Samuel |
Location: | LIBSB 108 |
Features: | - Equipped with a tungsten filament emitter;
- Operates at accelerating voltage at 40 kV - 125 kV; - Achieves lattice resolution of 0.144nm and particle image resolution of 0.35nm at 125 kV; - Embedded with bottom-mount CCD; - Allows ± 30 degrees image rotation and montage imaging. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 109 |
|
|
Features: | The Microscope
- Equipped for cold field emission; - Operates at accelerating voltage at 0.5 - 30 kV; and beam deceleration at 0.1 – 2.5 kV; - Image resolution : 1.0nm at 15kV; 1.3nm at 1kV; - Fitted with 2 secondary electron detectors (upper & lower); can perform SE/BSE signal mixing function; - Imaging magnifications: The EDS system - Equipped with electronically cooled silicon drift detector; - Sensor active area: 30 mm2; - Resolution: 128eV/Be-Pu; - For qualitative and semi-quantitative micro-analysis of elements on material and biological samples; - Iridium Ultra Premium software package features spectra, mapping imaging and automation analysis tools. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 109 |