Features: |
White confocal combines a white light laser source, tunable excitation filtering, tunable beam-splitting and tunable emission-detection – with full access to the keys for all colors. It allows users to specify any excitation wavelength between 470nm and 670nm. Together with an AOBS (acoustical optical beam splitter) up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. Because the WLL system is a pulsed laser it can be used in conjunction with hybrid detectors to provide gated suppression (removal) of auto-fluorescence and reflected light. The SP8's spectral detection system uses a prism spectrometer based emission separation (not filters) that allows user selection of emission detection bandwidth as well as spectral scanning of emission profiles. This allows users to precisely select any emission band-pass they require. Additionally, the system can scan at 8000Hz for ultra-fast scanning (and greatly reduced photo-bleaching) via its selectable resonant scanner. It is fully enclosed for heating and environmental control with media perfusion capabilities and CO2 control. The system is ideal for imaging live cells, tissue in culture dishes and chambered cover glass (#1.5). Microscope
An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light. An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.
Reflection/fluorescence mode (spectral detection range 400-800 nm)
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Staff-in-charge: |
Anny Cheung |
Back-up Support: | Josie Lai |
Location: | LIBSB 108A |
Remarks: |
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. |
Features: | - Equipped for fixed and live cell imaging, time-lapse imaging, multi-fluorescence z-stack 3-D, FRET/FRAP, SIM/HSD, multi-position time-lapse and mosaic outline imaging;
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Staff-in-charge: |
- Josie | ||||||||||||||||||
Back-up Support: | - Anny/Samuel | ||||||||||||||||||
Location: | LIBSB 108B | ||||||||||||||||||
Remarks: | For SBS Experienced and Trained user only.
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. - Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
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Features: | - Equipped for fixed and live cell imaging, time-lapse imaging and multi-fluorescence z-stack 3-D imaging;
- Connected to an IX81 inverted microscope with objectives 4x, 10x, 20x, 40x, 63x oil and epi-fluorescence filters for DAPI, FITC and TRITC; - Incubation chamber for live cell imaging; - Laser sets of spectral type multi-Argon (458, 488, 515) LD405, LD559 and LD635. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 108B |
Remarks: | For SBS Experienced and Trained user only.
- Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice. - Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
Features: | - Equipped for fixed/live cell imaging; time-lapse imaging and high-speed multi-fluorescence optical sectioning;
- Connected to Leica DMI6000 inverted microscope with fluorescence filters for I3 (Ex 450-490 / Em LP515) and N2.1 (Ex 515-560 / Em LP590); - Laser sets multi-Argon 458, 476, 488, 514, DPSS 561 and HeNe633; - Objectives: 10x, 20x, 40x, 40x oil, 63x water, 100x oil. |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Remarks: | - Data files stored on the machine will be deleted FIVE working days after experiments without advanced notice.
- Data files greater than 1GB will be automatically removed TWO working days after experimentation. |
Features: |
- Equipped for fixed and live cell imaging
Microscope • UPLSAPO4X, N.A. 0.16, WD 13 Filter sets: DAPI/Hoechst, FITC/EGFP, PI/TRITC, Cy5 Light source: X-Cite 120Q Metal halide or CoolLED pE4000 UV with 16 Selectable wavelengths including 365, 385, 405, 435, 460, 470, 490, 500, 525, 550, 580, 595, 635, 660, 740, 770nm Software interface: CellSens Dimension Camera: DP80 Double-CCD
Monochrome CCD: |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Features: | Microscope
- Zoom range: - Objectives: Filter sets: DAPI, GFP, Cy3, Cy5 Software interface: CellSens Camera: DP80 Double-CCD Color CCD: Resolution at 12.5 megapixels (4080 x 3072) Monochrome CCD: |
Staff-in-charge: |
- Josie |
Back-up Support: | - Anny / Samuel |
Location: | LIBSB 110 |
Features: |
Imaris is Bitplane's core scientific software module that delivers all the necessary functionality for data visualization, segmentation, analysis and interpretation of 3D and 4D microscopy datasets.
Modules available:
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Staff-in-charge: |
- Josie |
Back-up Support: | - Anny/Samuel |
Location: | LIBSB 108 |