- Ion source: (i) Jet Stream Electron Spray Ionization (ESI) - for charged, polar compounds; mass range m/z 5-3000 (ii) Atmospheric-pressure Chemical Ionization (APCI) – for uncharged, low-polarity compounds.

- Standard columns: (i) RRHD C18 column, 2.1 x 100mm, 1.8 micron (ii) RRHD C18 column, 2.1 x 50mm, 1.8 micron (iii) RRHT C18 column, 2.1 x 50mm, 1.8 micron, 600Bar

- Autosampler : Sample capacity up to 108 of 2ml vials (in 2 x 54 vial plate) ; Injection range : 0.1-20ul in 0.1ul increments

- Diode Array Detector (DAD): Wavelength range 190 – 640nm (wavelength accuracy 1nm)

- MassHunter MS Optimizer software - automatically optimizes ion transitions and fragmentor and collision energies for both singly charged small molecules

Staff-in-charge:

- Not open to non-SBS users.

- Please contact Dr. Suen in person for discussion and booking.

- General guidelines on use of Agilent 6460 Triple Quad LC/MS

- Application & Sample Submission form

- Broadband mass resolving power up to 40,000 and 1 ppm mass accuracy for highest confidence.

- Scanning speed: 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode

- LID-LIFT process delivers MS/MS spectra with nominal mass resolution for peptides.

- Laser-irradiation self-cleaning Ion source: ensures robust, long-term highest-performance operation.

Staff-in-charge:

- For SBS MEMBERS ONLY.

- General guidelines on use of Bruker UltrafleXtreme MALDI-TOF-TOF Mass Spectrometry System

- Application & Sample Submission form

- Nano LC system designed to optimize low flow separations of protein / peptides for mass spectrometry to provide the best resolution, sensitivity, and selectivity for proteomics applications

- Associated with fraction collector Bruker Proteineer fcII to fractionate and spot fractions onto MALDI AnchorChip targets plate for MALDI TOF TOF protein identification

- Trap column: facilitate pre-concentration and cleaning of sample

- Low flow rate : very low flow rates possible, down to 20 nL/min

- Maximum pressure: available up to 620 bar

- Injection cycle times as low as 15 s

Bruker Proteineer fc II

- As a LC-MALDI fraction collector offline coupling of liquid chromatography (LC) to MALDI mass spectrometry (LC-MALDI)

- The system collects separated peptide samples from Dionex Nano- LC systems and deposits them on MALDI AnchorChip targets for MALDI TOF TOF protein identification

- Simultaneous preparation of both LC flow and supporting matrix solution enabled by sheath flow

- Four positions in MTP format for MALDI Anchor Targets or well plates and a position for matrix or washing solution.

- Fraction collection time: > 7 sec./fraction

Staff-in-charge:

- 2X NGC F10 pump modules for a flow rate of 0.001–10 ml/min at 3,650 psi (252 bar, 25.2 MPa)

- 1X Sample pump for application of large volume of sample

- NGC multi-wavelength detector module for simultaneous four-wavelength detection at 190–800 nm, for high-accuracy detection of proteins, peptides, and nucleic acids

- pH and conductivity detectors

- BioFrac fraction collector for up to 180 samples

ChromLab™ Software provides powerful system control, automated methods, and analysis options

Staff-in-charge:

- Not open to non-SBS users.

- New users please contact Dr. Suen in person for discussion before start

2. Guidelines on Core Chromatography Column Usage

3. Column Request Form

- Method optimization – Optimal purification condition can be determined in an automated systematic manner using Scouting Wizard in ChromLab software

- Multi-step / Tandem Purification – Multi-step purification templates offer optimal efficiency for the tandem purification in multidimensional chromatography strategies or sequential purification of samples in multiple columns

- 2X NGC F10 pump modules for a flow rate of 0.001–10 ml/min at 3,650 psi (252 bar, 25.2 MPa)

- 1X Sample pump for application of large volume of sample

- NGC multi-wavelength detector module for simultaneous four-wavelength detection at 190–800 nm, for high-accuracy detection of proteins, peptides, and nucleic acids

- pH and conductivity detectors

- 1X Buffer blending valve

- ChromLab™ Software provides powerful system control, automated methods, and analysis options

Staff-in-charge:

- Not open to non-SBS users.

- New users please contact Dr. Suen in person for discussion before start

2. Guidelines on Core Chromatography Column Usage

3. Column Request Form

- The Labconco CentriVap Vacuum Concentrators uses a combination of centrifugal force,

   vacuum and heat to speed evaporation of multiple small samples in applications such as molecular biology,

   proteomics, genomics and cell biology.

Specifications and features of Labconco Centrifugal Concentrator

Staff-in-charge:

- Not open to non-SBS users.

- New users please contact Dr. Suen in person for use

- Guidelines on usage of Labconco Centrifugal Concentrator

- SpeedVac service request form